Verwijscase SDU: Corneal reconstruction


Two patients, a dog and a cat, presented with a corneal ulcer that was progressive and had been treated with medication by the referring veterinarians but did not respond to therapy. The patients were referred for diagnostics and treatment. I recommended corneal reconstruction with a CLCT graft (a corneo-limbo-conjunctival transposition graft, also known as a CCT or corneoconjunctival transposition) in each case and the treatment led to resolution of clinical signs. One of the patients was found to also have dry eye (the dog) and went on long term treatment with Optimmune.
Several points to remember that are extrapolated from several recent studies include:

Further reading (enter the title in to read the abstract):

Sanchez RF, Innocent G, Mould J, Billson FM. Canine Keratoconjunctivitis Sicca: disease trends in a review of 229 cases. Journal of Small Animal Practice 2007; 48(4): 211-217

O’Neill DG, Lee MM, Brodbelt DC, Church DB, Sanchez RF. Corneal ulcerative disease in dogs under primary veterinary care in England: epidemiology and clinical management. Canine Genetics and Epidemiology. 2017; 4(10): 171.

Graham KL, White JD, Billson FM. Feline Corneal sequestra: outcome of corneoconjunctival transposition in 97 cats (109 eyes). Journal of Feline medicine and Surgery 2017;19(6):710-716.

Cebrian P, Escanilla N, Lowe RC, Dawson C, Sanchez RF. Corneo-limbo-conjunctival transposition to treat deep and perforating corneal ulcers in dogs: a review of 418 eyes and corneal clarity scoring of 111 eyes. Veterinary Ophthalmology 2020; 00: 1– 11 (early online view)


Rick F Sanchez BSciBiol, DVM, CertVOphthal, DipECVO, FHEA
EBVS accredited, European Specialist in Veterinary Ophthalmology


A dog: Kelly, Bulldog, 1 year old female
A cat: Mittens, exotic, 3 year old male


Kelly: red eyes for an undetermined period of time, small amount of mucoid discharge also in both eyes, that were treated with a topical antibiotic (CAF) until the right eye (OD) developed a central, small, but very painful corneal ulcer.

Mittens: sudden development of a corneal ulcer of unknown origin in the right eye. Treated with a topical antibiotic (CAF for a few days and later Soligental) but it did not respond to therapy and continued to deteriorate.

Medical imaging





Kelly: the first image shows the findings described below. The second image shows the CLCT graft approximately 2 weeks after surgery, with a suture reaction around the microsutures. Corneal edema and lack of transparency of varying degrees are normal at that stage. The third image shows Kelly, seen from a distance. The right eye has already healed, Kelly has no redness or mucoid discharge, she is comfortable and the pupillary axis (vision axis) shows no scarring (there is good vision).





Mittens: the first image shows the findings described below. The second image shows the CLCT graft approximately 2 weeks after surgery with very little suture reaction and the expected vascularization. Note that the corneal section and the conjunctival section are very similar in level of transparency. The third image shows the eye once the sutures have completely dissolved. The corneal section of the graft is completely clear so the patient can see very well. The conjunctival section (medioventrally) is more opaque, which is expected, but still transmits light. In between is a visible white line, the limbus, which is also expected.


The significant findings of the ophthalmic exam included the following.

Kelly: ocular pain (blepharospasm) OD only, mild to moderate conjunctival hyperemia OU (OD>OS), small amount of mucoid discharge OS only, distichiasis upper and lower eyelid OU, corneal edema OD with a centrally located small descemetocele (a deep, cratered ulcer, with a clear center that did not stain with fluorescein). STT1 readings: 16 mm/min OD (painful eye) and 10mm/min OS (ref. ≥15mm/min in the absence of clinical signs consistent with dry eye and/or pain).

Mittens: ocular pain (bepharospasm), moderate to marked conjunctival hyeremia, large and deep (75%) corneal ulcer of unknown origin OD affecting the central cornea and accompanied by stromal melting (i.e. irregular corneal surface and mucus-like surface), corneal edema and vascularization.


Kelly: bilateral distichiasis, primary dry eye (KCS, bilateral) with an acute ulcerative presentation (OD) that has deteriorated into a descemetocele.

Mittens: deep, melting corneal ulcer of unknown origin (and affected by the ‘brachycephalic factor’).

Treatment & therapy

Corneal reconstruction with CLCT in each case, followed by medical therapy consisting on a topical antibiotic (chloramphenicol drops, combined with gentamicin drops in the cat) until healing of the graft, oral NSAID once daily for 7-14 days, short term use (1 day in the cat and 3 days in the dog) of atropine drops to help control ocular discomfort and a protective collar. The canine patient (Kelly) also underwent distichiasis removal with ‘blend’ electrolysis (electrolysis/diathermy) under the same anesthetic and started life-long use of Optimmune 2x day that was later reduced to 1x day.